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rhccl21  (R&D Systems)


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    R&D Systems rhccl21
    Rhccl21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhccl21/rhccl20/pm34197491-73-34-35
    Average 90 stars, based on 1 article reviews
    rhccl21 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Human beta-defensin 3 induces maturation of human langerhans cell-like dendritic cells: an antimicrobial peptide that functions as an endogenous adjuvant.
    Article Snippet: The following cytokines and chemokines were purchased from R&D Systems (Minneapolis, MN): rhGM-CSF, rhIL-4, rhTGF-b1, rhTNF-a, rhCCL19, and rhCCL21.

    Article Title: Human Beta Defensin 3 induces maturation of human langerhans cell like dendritic cells: An antimicrobial peptide that functions as an endogenous adjuvant
    Article Snippet: The following cytokines and chemokines were purchased from R&D Systems (Minneapolis, MN): rhGM-CSF, rhIL-4, rhTGF-β1, rhTNF-α, rhCCL19, and rhCCL21.

    Chemotaxis Assay:

    Article Title: Unexpected impairment of TNF-α-induced maturation of human dendritic cells in vitro by IL-4
    Article Snippet: .. The chemotaxis of IL-4 − and IL-4 + DC to rhCCL21 and rhCCL19 (R&D Systems) was assayed in 24-well cell culture plates with bare 8.0-μm-pore polycarbonate cell-culture inserts (Corning BV, Schiphol-Rijk, The Netherlands). ..

    Article Title: A humanized monoclonal antibody against the endothelial chemokine CCL21 for the diagnosis and treatment of inflammatory bowel disease
    Article Snippet: Human PB T-cells acclimated to 37°C were suspended in prewarmed RPMI (37°C) with 0.5% bovine serum albumin (BSA; Sigma-Aldrich; St. Louis, MO). .. Costar 24-well transwell plates with 6.5 mm diameter inserts with 5.0 μm pores (Sigma-Aldrich, St. Louis, MO) were prepared by placing 650 μl of prewarmed RPMI with 0.5% BSA that contained 0, 1200 ng/mL rhCCL21 (R&D Systems, Minneapolis, MN) or 1200ng/mL rhCCL21 pretreated for 1 hour with sample clones #7–39 in the bottom well and allowing plates to acclimate at 37°C for half an hour prior to chemotaxis assay. ..

    Cell Culture:

    Article Title: Unexpected impairment of TNF-α-induced maturation of human dendritic cells in vitro by IL-4
    Article Snippet: .. The chemotaxis of IL-4 − and IL-4 + DC to rhCCL21 and rhCCL19 (R&D Systems) was assayed in 24-well cell culture plates with bare 8.0-μm-pore polycarbonate cell-culture inserts (Corning BV, Schiphol-Rijk, The Netherlands). ..

    Clone Assay:

    Article Title: A humanized monoclonal antibody against the endothelial chemokine CCL21 for the diagnosis and treatment of inflammatory bowel disease.
    Article Snippet: Human PB T-cells acclimated to 37 ̊C were suspended in prewarmed RPMI (37 ̊C) with 0.5% bovine serum albumin (BSA; Sigma-Aldrich; St. Louis, MO). .. Costar 24-well transwell plates with 6.5 mm diameter inserts with 5.0 μm pores (Sigma-Aldrich, St. Louis, MO) were prepared by placing 650 μl of prewarmed RPMI with 0.5% BSA that contained 0, 1200 ng/mL rhCCL21 (R&D Systems, Minneapolis, MN) or 1200ng/mL rhCCL21 pretreated for 1 hour with sample clones #7–39 in the bottom well and allowing plates to acclimate at 37 ̊C for half an hour prior to chemotaxis assay. ..

    Article Title: A humanized monoclonal antibody against the endothelial chemokine CCL21 for the diagnosis and treatment of inflammatory bowel disease
    Article Snippet: Human PB T-cells acclimated to 37°C were suspended in prewarmed RPMI (37°C) with 0.5% bovine serum albumin (BSA; Sigma-Aldrich; St. Louis, MO). .. Costar 24-well transwell plates with 6.5 mm diameter inserts with 5.0 μm pores (Sigma-Aldrich, St. Louis, MO) were prepared by placing 650 μl of prewarmed RPMI with 0.5% BSA that contained 0, 1200 ng/mL rhCCL21 (R&D Systems, Minneapolis, MN) or 1200ng/mL rhCCL21 pretreated for 1 hour with sample clones #7–39 in the bottom well and allowing plates to acclimate at 37°C for half an hour prior to chemotaxis assay. ..



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    Purified T cells (5 × 10 5 cells/well) were added to upper chambers of Transwell plates. rhCCL20 (200 ng/ml), rhCXCL16 (200 ng/ml), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), <t>rhCCL21</t> (200 ng/ml), and PBS as a negative control were added to lower chambers. Plates were incubated for 90 minutes at 37°C. Migrated cells were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs and then counted using Neubauer chamber and flow cytometer. Migration index was calculated by the number of cells that migrated in the presence of each chemokine divided by the number of cells that migrated in the presence of PBS. The migration index of MAIT cells was normalized to the migration index of T cells. Values are expressed as the mean ± SEM. Data were obtained from 5 HCs. *, P < 0.05, **, P < 0.005 by paired t test.
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    rhccl21  (R&D Systems Hematology)
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    Purified T cells (5 × 10 5 cells/well) were added to upper chambers of Transwell plates. rhCCL20 (200 ng/ml), rhCXCL16 (200 ng/ml), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), <t>rhCCL21</t> (200 ng/ml), and PBS as a negative control were added to lower chambers. Plates were incubated for 90 minutes at 37°C. Migrated cells were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs and then counted using Neubauer chamber and flow cytometer. Migration index was calculated by the number of cells that migrated in the presence of each chemokine divided by the number of cells that migrated in the presence of PBS. The migration index of MAIT cells was normalized to the migration index of T cells. Values are expressed as the mean ± SEM. Data were obtained from 5 HCs. *, P < 0.05, **, P < 0.005 by paired t test.
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    Image Search Results


    Purified T cells (5 × 10 5 cells/well) were added to upper chambers of Transwell plates. rhCCL20 (200 ng/ml), rhCXCL16 (200 ng/ml), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), rhCCL21 (200 ng/ml), and PBS as a negative control were added to lower chambers. Plates were incubated for 90 minutes at 37°C. Migrated cells were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs and then counted using Neubauer chamber and flow cytometer. Migration index was calculated by the number of cells that migrated in the presence of each chemokine divided by the number of cells that migrated in the presence of PBS. The migration index of MAIT cells was normalized to the migration index of T cells. Values are expressed as the mean ± SEM. Data were obtained from 5 HCs. *, P < 0.05, **, P < 0.005 by paired t test.

    Journal: Oncotarget

    Article Title: Clinical relevance of circulating mucosal-associated invariant T cell levels and their anti-cancer activity in patients with mucosal-associated cancer

    doi: 10.18632/oncotarget.11187

    Figure Lengend Snippet: Purified T cells (5 × 10 5 cells/well) were added to upper chambers of Transwell plates. rhCCL20 (200 ng/ml), rhCXCL16 (200 ng/ml), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), rhCCL21 (200 ng/ml), and PBS as a negative control were added to lower chambers. Plates were incubated for 90 minutes at 37°C. Migrated cells were stained with APC-Alexa Fluor 750-conjugated anti-CD3, FITC-conjugated anti-TCR γδ, APC-conjugated anti-TCR Vα7.2 and PE-Cy5-conjugated anti-CD161 mAbs and then counted using Neubauer chamber and flow cytometer. Migration index was calculated by the number of cells that migrated in the presence of each chemokine divided by the number of cells that migrated in the presence of PBS. The migration index of MAIT cells was normalized to the migration index of T cells. Values are expressed as the mean ± SEM. Data were obtained from 5 HCs. *, P < 0.05, **, P < 0.005 by paired t test.

    Article Snippet: Recombinant human (rh) CCL20 (200 ng/ml; PeproTech, London, UA), rhCXCL16 (200 ng/ml; PeproTech), mixture of rhCCL20 and rhCXCL16 (200 ng/ml), rhCCL21 (200 ng/ml; PeproTech) and PBS were added to lower chambers containing serum free RPMI1640 media.

    Techniques: Purification, Negative Control, Incubation, Staining, Flow Cytometry, Migration